The forests of the watershed are primarily deciduous hardwoods with coniferous stands of hemlock and pine interspersed. The watershed is home to a variety of wildlife such as whitetail deer, black bear, wild turkey, songbirds, raptors, waterfowl, rodents, fox, raccoon, opossum, skunk, reptiles and amphibians. Common fish in the colder flowing stream segments are brook and brown trout while the warmer water reaches hold suckers, smallmouth bass and those species tolerant of warmer conditions. Half of all PA Fish and Boat Commission approved trout waters in Union County are in the Buffalo Creek Watershed.